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Phytochemical Profiling and Fluorescence Analysis of Aerial Parts of Vitis vinefera for Modern Health Care Systems

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Phytochemical Profiling and Fluorescence Analysis of Aerial Parts of Vitis vinefera for Modern Health Care Systems


Anita V. Handore | Sharad. R. Khandelwal

https://doi.org/10.31142/ijtsrd2513



Anita V. Handore | Sharad. R. Khandelwal "Phytochemical Profiling and Fluorescence Analysis of Aerial Parts of Vitis vinefera for Modern Health Care Systems" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-1 | Issue-6, October 2017, pp.262-270, URL: https://www.ijtsrd.com/papers/ijtsrd2513.pdf

In the modern health care system, phytochemical rich plants have widespread applications. Since the ancient times, V.vinefera has been extensively used in various ayurvedic preparations due to presense of different bioactive compounds. However, its phyto-contituents shows variation on basis of variety and different parts of plant .The aim of this research is to study the phytochemical profiling and florescence analysis of aerial parts by use of distinct varieties of Vitis vinefera. Healthy aerial parts (leaf lamina, stem and petiole) of different black and white varieties of Vitis vinefera were randomly collected. Organic and aqueous extracts were prepared by soaking the shade dried powder (10%) of each part into 70% ethanol and sterile distill water separately. Phytochemical profiling and fluorescent analysis was carried out by use of reported methods with few modifications .Experimental evaluation showed high abundance of various bioactive phytochemicals in both organic as well as aqueous extracts. It was revealed that organic extracts of both varieties gave best results for some polyphenolic groups like flavonoids, ellagic acid, tannins as well as other groups like glycosides, alkaloids, diterpenes, coumarins, fats and oils,etc. .Only the test for anthocyanin was found to be negative in white variety. It was found that carbohydrates and reducing sugar, proteins and amino acids, saponins as well as tannins has given comparatively good results in aqueous extract. Findings of the fluorescence analysis demonstrated that powdered plant parts treated with different solvents and reagents has shown specific shades of green, brown, pink, black and yellow colour in visible and ultraviolet light. Whereas, some samples showed white, yellow and green fluorescence under short UV- light (254 nm) and long UV light (365 nm).Therefore, based on the outcome of this study, it can be concluded that almost all the aerial parts of V.vinefera are rich natural source of various bioactive phytochemicals with clinically proved therapeutic potential. Further investigation for isolation, characterization and purification of such phyto constituents from these cost effective natural sources may become basis for their promising application in the modern health care systems

Vitis vinefera , Chronic diseases Phytochemicals, Fluorescence, Bioactive , Aerial parts, Therapeutic agent


IJTSRD2513
Volume-1 | Issue-6, October 2017
262-270
IJTSRD | www.ijtsrd.com | E-ISSN 2456-6470
Copyright © 2019 by author(s) and International Journal of Trend in Scientific Research and Development Journal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0)

International Journal of Trend in Scientific Research and Development - IJTSRD having online ISSN 2456-6470. IJTSRD is a leading Open Access, Peer-Reviewed International Journal which provides rapid publication of your research articles and aims to promote the theory and practice along with knowledge sharing between researchers, developers, engineers, students, and practitioners working in and around the world in many areas like Sciences, Technology, Innovation, Engineering, Agriculture, Management and many more and it is recommended by all Universities, review articles and short communications in all subjects. IJTSRD running an International Journal who are proving quality publication of peer reviewed and refereed international journals from diverse fields that emphasizes new research, development and their applications. IJTSRD provides an online access to exchange your research work, technical notes & surveying results among professionals throughout the world in e-journals. IJTSRD is a fastest growing and dynamic professional organization. The aim of this organization is to provide access not only to world class research resources, but through its professionals aim to bring in a significant transformation in the real of open access journals and online publishing.

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