Home > Pharmacy > Pharmaceutics > Volume-1 > Issue-5 > Impact of Environmental Factors on Secondary Metabolite Diversity and Free Radical Scavenging Activity of V .vinefera (Jumbo Seedless) From Nashik Valley

Impact of Environmental Factors on Secondary Metabolite Diversity and Free Radical Scavenging Activity of V .vinefera (Jumbo Seedless) From Nashik Valley

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Impact of Environmental Factors on Secondary Metabolite Diversity and Free Radical Scavenging Activity of V .vinefera (Jumbo Seedless) From Nashik Valley


Anita V. Handore | Prof. Dr. Sharad R. Khandelwal

https://doi.org/10.31142/ijtsrd2240



Anita V. Handore | Prof. Dr. Sharad R. Khandelwal "Impact of Environmental Factors on Secondary Metabolite Diversity and Free Radical Scavenging Activity of V .vinefera (Jumbo Seedless) From Nashik Valley" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-1 | Issue-5, August 2017, pp.33-39, URL: https://www.ijtsrd.com/papers/ijtsrd2240.pdf

Environmental factors has great influence on biosynthesis of secondary metabolites in plants. Nashik valley is located at highest elevated point of the Maharashtra state of India . Thereby, vineyards of these area are always subjected to various stressful environmental conditions. Present work aimed to study the effect of environmental factors on secondary metabolite diversity and free radical scavenging activity of different aerial parts of V.Vinefera from Nashik valley. Organic extracts are prepared by soaking the dry powder (10%) of different aerial parts viz.leaf lamina, stem and petiole of Jumbo seedless cultivar into 90% Methanol. Presence of different secondary metabolites were confirmed by phytochemical analysis. Detection of spots of secondary metabolites was observed by Thin layer chromatography (TLC) using the solvent system, N-Butanol: Acetic Acid: Water (4:1:5). Folin-Ciocalteau method was used to determine total phenolic content whereas, total flavonoid content was estimated by aluminum chloride colorimetric assay. Free radical scavenging activity of extract was carried out by DPPH assay .Findings of study showed that almost all the aerial parts of V.vinefera are rich source of secondary metabolites with medicinal values. Results of TLC showed presence of different spots of secondary metabolites. Rf value of flavonoid –quercetin was found to be 0.85. Total phenolic content of petiole was found to be highest (0.92± 0.10) whereas leaf lamina showed lowest amount viz. 0.5± 0.07mg GAE/g The total flavonoid content of petiole was found to be 0.28±0.12mg/g quercetin equivalent which was highest in comparison to stem (0.13 ±0.04quercetin equivalent) and leaf lamina (0.11±0.01) mg/g quercetin equivalent. Satisfactory antioxidant activity (Radical scavenging activity) of different aerial parts were found in the order as, Stem (70.01%)>Petiole (67.00%)> leaf lamina (52.26%) respectively. The results showed positive linear correlation between total phenolic content and total flavonoid content of different aerial parts of black cultivar of V. vinefera. Therefore, our study emphasizes that environmental conditions of Nashik valley is significantly suitable for biosynthesis of various bioactive secondary metabolites in V.vinefera.

Climate change, environmental factors, Vitis vinefera, Secondary metabolites, Antioxidant


IJTSRD2240
Volume-1 | Issue-5, August 2017
33-39
IJTSRD | www.ijtsrd.com | E-ISSN 2456-6470
Copyright © 2019 by author(s) and International Journal of Trend in Scientific Research and Development Journal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0)

International Journal of Trend in Scientific Research and Development - IJTSRD having online ISSN 2456-6470. IJTSRD is a leading Open Access, Peer-Reviewed International Journal which provides rapid publication of your research articles and aims to promote the theory and practice along with knowledge sharing between researchers, developers, engineers, students, and practitioners working in and around the world in many areas like Sciences, Technology, Innovation, Engineering, Agriculture, Management and many more and it is recommended by all Universities, review articles and short communications in all subjects. IJTSRD running an International Journal who are proving quality publication of peer reviewed and refereed international journals from diverse fields that emphasizes new research, development and their applications. IJTSRD provides an online access to exchange your research work, technical notes & surveying results among professionals throughout the world in e-journals. IJTSRD is a fastest growing and dynamic professional organization. The aim of this organization is to provide access not only to world class research resources, but through its professionals aim to bring in a significant transformation in the real of open access journals and online publishing.

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