High Performance Liquid Chromatography (HPLC)

Panjak Chasta; Dr Gaurav Kumar Sharma | Dr. Kausal Kishore Chandrul; Mohd Ali

Title:
High Performance Liquid Chromatography (HPLC)

Authors:
Mohd Ali | Panjak Chasta | Dr Gaurav Kumar Sharma | Dr. Kausal Kishore Chandrul

Cite This Article :

Mohd Ali | Panjak Chasta | Dr Gaurav Kumar Sharma | Dr. Kausal Kishore Chandrul "High Performance Liquid Chromatography (HPLC)" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5, August 2021, pp.1498-1501, URL: https://www.ijtsrd.com/papers/ijtsrd45146.pdf

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Abstract :
High Performance Liquid Chromatography (HPLC) is a process of separating components in a liquid mixture. A liquid sample is injected into a stream of solvent (mobile phase) flowing through a column packed with a separation medium (stationary phase). Sample components separate from one another by a process of differential migration as they flow through the column.As bands emerge from the column, flow carries them to one or more detectors which deliver a voltage response as a function of time. This is called a chromatogram. For each peak, the time at which it emerges identifies the sample constituent with respect to a standard. The peak’s area represents the quantity .HPLC provides a highly specific, reasonably precise, and fairly rapid analytical method for a plethora of complicated samples.This is difficult in detecting compounds. Low sensitivity of some compounds towards the stationary phase in the columns is difficult.

Keywords :
High-performance liquid chromatography (HPLC), Types, Advantages, Disadvantages

Publication Details:

Unique Identification Number : IJTSRD45146

Published In : Volume-5 | Issue-5, August 2021

Page Number(s) : 1498-1501

Publisher Name : IJTSRD | www.ijtsrd.com | E-ISSN 2456-6470

Copyright © 2019 by author(s) and International Journal of Trend in Scientific Research and Development Journal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0)

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