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Bacterial Endotoxin Test by Gel-Clot Method

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Bacterial Endotoxin Test by Gel-Clot Method


Faiz Hashmi | Ankush Thakur

https://doi.org/10.31142/ijtsrd22945



Faiz Hashmi | Ankush Thakur "Bacterial Endotoxin Test by Gel-Clot Method" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-3, April 2019, pp.564-567, URL: https://www.ijtsrd.com/papers/ijtsrd22945.pdf

The Bacterial Endotoxin Test (BET) is a test to quantify Endotoxin from ‘Gram-negative bacteria using ‘amoebocyte lysate’ extracted from ‘Limulus polyphemus or Tachypleus tridentatus’ i.e. horseshoe crab. Three techniques are used for the confirmation of endotoxins, those are: the gel-clot technique, the turbidimetric technique, and the chromogenic technique. Endotoxins are toxic complexes which is invariably associated with the cell-wall of the Gram-negative bacteria irrespective of bacterial pathogenicity. Endotoxins are rarely fatal, but they cause fever and hence Endotoxin carrying bacteria are known as ‘Pyrogen’. The test can be done by any of these three techniques. Among all, the gel-clot method is chosen to be superior. The test is carried out in a manner to avoid endotoxins contamination. Mostly bacterial Endotoxin test are performed for the inject-able pharmaceutical products, e.g. SWFI (Sterile Water for Injection), Normal Saline (Sodium Chloride Sterile) and other injections like Amikacin sulphate, Ascorbic acid, etc. Endotoxin had been authenticated as inflammatory agent, onset clinical diabetes, obesity, nausea, vomiting, diarrhea, fever, etc. Endotoxins are thus very necessary to get removed out from the inject-able pharmaceutical products.

Endotoxin, Gram-negative bacteria, Amoebocyte lysate, Lysate validation, Lysate reconstitution, Maximum Valid Dilution, Endotoxin Limit, Gel-clot technique


IJTSRD22945
Volume-3 | Issue-3, April 2019
564-567
IJTSRD | www.ijtsrd.com | E-ISSN 2456-6470
Copyright © 2019 by author(s) and International Journal of Trend in Scientific Research and Development Journal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0)

International Journal of Trend in Scientific Research and Development - IJTSRD having online ISSN 2456-6470. IJTSRD is a leading Open Access, Peer-Reviewed International Journal which provides rapid publication of your research articles and aims to promote the theory and practice along with knowledge sharing between researchers, developers, engineers, students, and practitioners working in and around the world in many areas like Sciences, Technology, Innovation, Engineering, Agriculture, Management and many more and it is recommended by all Universities, review articles and short communications in all subjects. IJTSRD running an International Journal who are proving quality publication of peer reviewed and refereed international journals from diverse fields that emphasizes new research, development and their applications. IJTSRD provides an online access to exchange your research work, technical notes & surveying results among professionals throughout the world in e-journals. IJTSRD is a fastest growing and dynamic professional organization. The aim of this organization is to provide access not only to world class research resources, but through its professionals aim to bring in a significant transformation in the real of open access journals and online publishing.

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