Soft tissue is removed from bones before they are macerated, bleached, and labelled. As most medical colleges do not handle bones after the dissection of human cadavers, a significant storehouse of human bones is lost in the absence of a regulated approach. Therefore, the goal of the current investigation was to govern the least time-consuming and most efficient way to prepare bones from embalmed and wet specimens. Bony parts have different nature like shaft of long bones require less time to dissolve and end part of long bone require more time to dissolve. The procedure utilised included a maceration step, which includes removing soft tissue before boiling the bones (according to porous nature of bones) in 60 litres of water for two hours. After 30 minutes of starting the boiling process, potassium hydroxide pellets (caustic potash, mol. wt. 56.11), weighing 200–250 gm for male bones and 150–200 gm for female bones, were added to speed up the maceration process. After the bones had finished macerating, they were bleached by immersing them in a 30–35 litre solution of hydrogen peroxide (30% w/v) (mol. wt. 34.01) for 12–14 hours. The bleached bones were then thoroughly cleaned with water before being degreased for 12 hours by soaking in 30 to 35 litres of extra pure acetone (boiling point 55.5° to 56°C). lacquer thinner after they had naturally dried by being spread out on blotting paper. lacquer thinner re. This study found that the aforementioned technique was quick, easy, odourless, and produced high-quality human bone. This investigation came to the conclusion that the above approach was efficient, quick, odourless, and produced good quality human bones for anatomical examination. the excision of soft tissues Bleaching, degreasing, maceration, and potassium hydroxide.
Bone, Maceration
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