There are many situations where human activities have significant effect on the environment, ozone layer damage is one of them. This paper aims to investigate into the knowledge and attitude of the staff of Emmanuel Alayande College of Education, Oyo State, Nigeria, on the use of ozone layer depleting substances. Data in the study was collected via a questionnaire divided into two parts; the first part is designed to determine the knowledge of the respondents about the ozone layer as well as elucidate common misconceptions several people have about the ozone layer and related concepts such as greenhouse effect and global warming and the second part was constructed to determine the attitude of the respondents towards the use of ozone layer depleting substances. Two hypotheses were formulated and tested at 0.05 level of significance, the results of the data obtained were analysed using percentages and chi-square statistical tool of Statistical Package for Social Sciences (SPSS). The results showed that the knowledge of the staff in the field of science supersedes those of other disciplines, yet they have a few common misconceptions. Also, it was found that the attitude of the staff is significantly influenced by their level of education and area of academic discipline, as the null hypothesis formulated was rejected. It was recommended that environmental studies should be included in the school curriculum from primary level to tertiary level in order to enhance the knowledge of the populace on ozone layer depletion and its consequences.
                                
                                
                                    
                                    Knowledge, attitude, ozone
                                
                                
                                
                                
                                    
                                        
                                        
                                        
                                        
                                            
                                                
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