The study was aimed to determine the impact of Annona muricata on biochemical and histological profile on alcohol-induced testicular dysfunction in male Wistar Rat. Twenty-five male Wistar rats with a weight of 130-160 grams were employed. Group A received feed and distilled water only, group B received 40-ml of Alcohol, and group C received 200mg/kg of ethanolic leaf extract of Annona muricata (ELAM). Groups D and E received 40-ml of Alcohol and were treated with 200mg/kg and 400mg/kg of ELAM for three weeks. The administration was done through oral gavage daily for three weeks. Data obtained for testosterone, Malondialdehyde (MDA), Catalase (CAT), gonado-somatic index, and semen quality (total sperm count, motility, and morphology) were analyzed using ANOVA followed by post hoc LSD, and values were considered significant at p<0.05. The results showed a significant (p<0.05) decrease in testosterone level, total sperm count, activity motility, and normal sperm cells in-group B compared to A. However, in treatment groups, Annona muricata attenuated the effect on the parameters above, with a significant (p<0.05) increase compared to group B. Alcohol toxicity caused an increase in abnormal sperm cells and gonado-somatic index of the testes significantly (p<0.05), which was attenuated by the Annona muricata. Also, significant higher MDA activities were observed in group B; with no significant change in groups, C to E. CAT activities showed a significant decrease in group B and significantly attenuated CAT activities in-group E. Histoarchitectural results revealed spermatogenic arrest and necropsied spermatids in group B, while moderate healing and increased spermatogenic process by the extracts in the treated groups. The study revealed testicular dysfunction resulting in infertility by alcohol toxicity; however, Annona muricata showed improved fertility after reduced testicular function and improved CAT activities based on dose-dependent with no effect on MDA level.
Alcohol, Annona muricata, Male infertility, Oxidative stress, Antioxidants, semen quality, testicular dysfunction
International Journal of Trend in Scientific Research and Development - IJTSRD having
online ISSN 2456-6470. IJTSRD is a leading Open Access, Peer-Reviewed International
Journal which provides rapid publication of your research articles and aims to promote
the theory and practice along with knowledge sharing between researchers, developers,
engineers, students, and practitioners working in and around the world in many areas
like Sciences, Technology, Innovation, Engineering, Agriculture, Management and
many more and it is recommended by all Universities, review articles and short communications
in all subjects. IJTSRD running an International Journal who are proving quality
publication of peer reviewed and refereed international journals from diverse fields
that emphasizes new research, development and their applications. IJTSRD provides
an online access to exchange your research work, technical notes & surveying results
among professionals throughout the world in e-journals. IJTSRD is a fastest growing
and dynamic professional organization. The aim of this organization is to provide
access not only to world class research resources, but through its professionals
aim to bring in a significant transformation in the real of open access journals
and online publishing.