Background: The main pathophysiology of diabetes mellitus (DM) is oxidative stress, which is an imbalance between production of free radicals and antioxidant system in the body. Persistent hyperglycemia causes increased production of free radicals especially reactive oxygen species (ROS), in tissues from glucose auto-oxidation and protein glycosylation. Aim: To investigate the comparative effect of Aloe vera gel, rind and a combination of gel and rind on the oxidative stress in alloxan-induced diabetic rats. Materials and Methods: 25 male wistar rats weighing between 220–260g were used for this study. They were divided into five groups (A - E) of 5 rats each. Group A served as positive control group and received distilled water only. Group B was the negative diabetic group with no extract administration. Groups C, D and E were diabetic groups that received 300mg/kg of Aloe vera rind, 300mg/kg of Aloe vera gel and combination of 150mg/kg of Aloe vera rind and 150 mg/kg of Aloe vera gel respectively. Extract administration was done orally, once daily for 14 days. At the end of administration, oxidative stress biomarkers were assessed. Results: There was significant decrease in SOD level in group B (9.87±0.20) when compared with the control (15.88±0.39). Conversely, groups C (17.50±0.35) and D (17.94±0.22) showed significant increase when compared with the control. However, group E showed no significant change when compared with the control (15.88±0.39). On the other hand, there was significant increase in SOD in control group A (15.88±0.39) and all the test groups when compared with group B (9.87±0.20). Similarly, a significant increase in MDA was observed in group B (5.39±0.20) when compared with the control (4.15±0.21). Groups C (3.62±0.20) and D (3.23±0.12) showed significant decrease when compared with control (4.15±0.21). Furthermore, group E (4.14±0.26) showed no significant difference when compared with the control (4.15± 0.2). On the other hand, groups A and all the test groups showed significant decrease in MDA when compared with group B (5.39±0.20). Conclusion:- Aloe vera gel and Aloe vera rind extracts singly appeared more potent in reducing oxidative stress in diabetic rats when compared to combined extracts of Aloe vera rind + gel at lower doses.
Diabetes mellitus, Oxidative stress, Aloe vera, Antioxidant
International Journal of Trend in Scientific Research and Development - IJTSRD having
online ISSN 2456-6470. IJTSRD is a leading Open Access, Peer-Reviewed International
Journal which provides rapid publication of your research articles and aims to promote
the theory and practice along with knowledge sharing between researchers, developers,
engineers, students, and practitioners working in and around the world in many areas
like Sciences, Technology, Innovation, Engineering, Agriculture, Management and
many more and it is recommended by all Universities, review articles and short communications
in all subjects. IJTSRD running an International Journal who are proving quality
publication of peer reviewed and refereed international journals from diverse fields
that emphasizes new research, development and their applications. IJTSRD provides
an online access to exchange your research work, technical notes & surveying results
among professionals throughout the world in e-journals. IJTSRD is a fastest growing
and dynamic professional organization. The aim of this organization is to provide
access not only to world class research resources, but through its professionals
aim to bring in a significant transformation in the real of open access journals
and online publishing.