In this study, brown seaweed (Sargassum sp.) was used to produce bioethanol by using enzymatic liquefaction and saccharification method. Bioethanol from brown seaweed (Sargassum sp.) was more commercial than using any other starch based raw materials because it can be easily collected on Chaung Tha beach in Myanmar without any impact on environment. In this regard, the productivity of bioethanol from brown seaweed (Sargassum sp.) was determined by separate hydrolysis and fermentation (SHF) with yeasts. Two types of yeasts were used. Saccharomyces cerevisiae was used for glucose fermentation in brown seaweed and selected nitrogen fixing yeast isolate (N3,N18,N24) were used for mannitol fermentation which consist plenty in brown seaweed. The effects of enzymatic liquefaction, enzymatic saccharification and fermentation on this sample were studied. From the fermentation studies, brown seaweed (Sargassum sp.) gave the ethanol percent by weight of 2.56% using Saccharomyces cerevisiae only and 4.1% by using mixture of yeast Saccharomyces cerevisiae and selected nitrogen fixing yeast isolate. The maximum yield of crude ethanol was 32.5% by fermentating yeast mixture of Saccharomyces cerevisiae and nitrogen fixing yeast isolate. When it was fermented by just only Saccharomyces cerevisiae, yield of crude ethanol percent was 20.3%.
Brown seaweed (Sargassum sp.), bioethanol, enzymatic liquefaction, saccharification, fermentation
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