Loaches (Cobitidae: Pisces) native to the Brahmaputra basin is a prominent group of ornamental fishes. Selected aspects of reproductive biology of three species of loaches viz., Botia dario, Acanthocobitis botia and Canthophrys gongota have been studied to provide basic information towards development of captive breeding protocol. All the three species were annual breeders, peak spawning season being observed during early monsoon. Relative fecundity of B. dario was obtained as 1051 (absolute fecundity between 9075 and 13890), that of A. botia was 885 (absolute fecundity ranged between 5690 and 6835) and 310 for C. gongota (absolute fecundity ranged from 4980 to 5358). Diameter of mature ova are found to be almost similar in all the three species of loaches, within the range of 0.60- 0.63. In all the cases linear relationships obtained were highly significant.
Fecundity, ova diameter, Botia dario, Acanthocobitis botia, Canthophrys gongota, upper Brahmaputra basin
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