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Analysis of DNA Content of Wild and Cultured Labeo Calbasu (Hamilton, 1822) Using Nanophotometer

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Analysis of DNA Content of Wild and Cultured Labeo Calbasu (Hamilton, 1822) Using Nanophotometer


Nisha Rana | Seema Jain

https://doi.org/10.31142/ijtsrd19105



Nisha Rana | Seema Jain "Analysis of DNA Content of Wild and Cultured Labeo Calbasu (Hamilton, 1822) Using Nanophotometer" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-1, December 2018, pp.906-909, URL: https://www.ijtsrd.com/papers/ijtsrd19105.pdf

Labeo calbasu is an important food fish and commonly known as the “calbasu” or “Black Rohu”. The present study was conducted on DNA extraction and determination of DNA quantity of both male and female of wild and cultured Labeo calbasu using Nanophotometer. DNA isolation was done and gel electrophoresis was carried out. Extracted DNA was analyzed using nanophotometer (Nanophotometer P330; Implen, Germany) to determine the concentration of DNA and its purity level. Several different methods have been employed in the quantification of nuclear DNA over the past 50 years. The ease of use of this technique not only makes it a feasible option for small volume analysis of DNA but also a practical alternative for spectrophotometric measurement. Significant progress was made to measure micro volume liquid samples (<1µl) in biotechnology & pharmaceutical applications. For the present study both male and female of wild Labeo calbasu (from middle Ganga Region) and cultured Labeo calbasu (from culture ponds and/ hatchery) were taken. The value of DNA concentration in female of wild Labeo calbasu was in between 58 -62 ng/µl and of male was in between range of 64- 68 ng/µl. The value of DNA concentration in female of cultured Labeo calbasu was 60- 66 ng/µl and of male 68- 74 ng/µl.

DNA Quantity, wild, cultured, Nanophotometer, Labeo calbasu


IJTSRD19105
Volume-3 | Issue-1, December 2018
906-909
IJTSRD | www.ijtsrd.com | E-ISSN 2456-6470
Copyright © 2019 by author(s) and International Journal of Trend in Scientific Research and Development Journal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0)

International Journal of Trend in Scientific Research and Development - IJTSRD having online ISSN 2456-6470. IJTSRD is a leading Open Access, Peer-Reviewed International Journal which provides rapid publication of your research articles and aims to promote the theory and practice along with knowledge sharing between researchers, developers, engineers, students, and practitioners working in and around the world in many areas like Sciences, Technology, Innovation, Engineering, Agriculture, Management and many more and it is recommended by all Universities, review articles and short communications in all subjects. IJTSRD running an International Journal who are proving quality publication of peer reviewed and refereed international journals from diverse fields that emphasizes new research, development and their applications. IJTSRD provides an online access to exchange your research work, technical notes & surveying results among professionals throughout the world in e-journals. IJTSRD is a fastest growing and dynamic professional organization. The aim of this organization is to provide access not only to world class research resources, but through its professionals aim to bring in a significant transformation in the real of open access journals and online publishing.

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