Aloe vera is a well-known medicinal plant and possesses many antimicrobial activities. The present research focuses on the phytochemical analysis of leaf extract and gel and assessing antibacterial and antifungal activity against Staphylococcus aureus, Bacillus subtillis, E. coli, Klebsiella pneumonia, Salmonella typhimurium, Pseudomonas aeroginosa, Proteus vulgaris, Candida albicans and Aspergillus niger. The plant samples were collected from Goniana mandi, Punjab, and the extract was prepared. The extract was further analyzed for physiochemical, phytochemical and antimicrobial susceptibility tests. Results have shown that plant possesses phenols, glycosides, terpenoids, carbohydrates, flavonoids, resins, tannins, sterols, and saponins. For 100mg of concentration Aloe vera, the zone of inhibition of test organisms Pseudomonas aeruginosa MTCC-2488 was 10.5mm, S.aureus MTCC-737 showed 14mm inhibition zone, E.coli showed 13 mm inhibition zone. Bacillus subtillis have shown the least inhibitory effect. Aspergillus niger MTCC-281 have shown inhibition zone of 11mm. Candida albicans shows an inhibition zone of 16mm by disc diffusion method and 4 mm by well diffusion method. S.aureus MTCC-737, Salmonella typhi, Kleibseilla sp., Proteus vulgaris have shown minimum inhibitory concentration of 25mm. Both fungal cultures Candida albicans and Aspergillus niger have shown MIC of 25mm. This study has shown that aloe vera leaf and gel complement one another in their medicinal properties. It can be an alternative to chemicals used in medications, food and cosmetic sectors.
Aloe vera, MIC, inhibition Zone, Gel, well diffusion
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