Lysine production in the broth culture of Bacillus species using carbohydrates as carbon source and seed meals as nitrogen source was investigated. Different carbohydrate and proteins seeds were sourced from an open market in Awka Anambra State South Eastern Nigeria and prepared in the laboratory using standard procedures. The carbohydrates(carbon source) and seed meals(nitrogen source) were added into Erlenmeyer flasks containing the basal medium and inoculated with different cultures of Bacillus subtilis PR13, B. subtilis PR9 and B. pumilus SS16. Maize hydrolysate recorded the highest reducing sugar(5.2mg/ml), followed by sorghum(4.8mg/ml) and the least was recorded by sweet potato(2.1mg/ml).The best carbon source for maximum lysine yield by B. subtilis PR13 was millet, while for B. subtilis PR9 and B. pumilus SS16 it was sorghum respectively. Maximum lysine production by B. subtilis PR13 was stimulated at a millet concentration of 6%, while enhanced lysine yield by B. subtilis PR9 and B. pumilus SS16 was observed at a sorghum concentration of 6%. The best nitrogen source for enhanced lysine yield by B. subtilis PR13 and B. pumilus SS16 was soyabean meal respectively, while for B. subtilis PR9 the best was peanut meal. Optimum lysine yield by B. subtilis PR13 and B. pumilus SS16 was observed at soyabean concentrations of 4% and 2% respectively, while maximum lysine accumulation by B. subtilis PR9 was observed at 4%. These findings indicate appreciable lysine production capability of Bacillus species when agricultural products are used as carbon and nitrogen sources.
Lysine production, broth, Bacillus species, carbohydrates, seed meals
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