Home > Biological Science > Biotechnology > Volume-2 > Issue-1 > RAPD based Molecular Characterization of Lavatera cachemiriana Cambess

RAPD based Molecular Characterization of Lavatera cachemiriana Cambess

Call for Papers

Volume-4 | Issue-5

Last date : 27-Aug-2020

Best International Journal
Open Access | Peer Reviewed | Best International Journal | Indexing & IF | 24*7 Support | Dedicated Qualified Team | Rapid Publication Process | International Editor, Reviewer Board | Attractive User Interface with Easy Navigation

Journal Type : Open Access

Processing Charges : 700/- INR Only OR 25 USD (for foreign users)

Paper Publish : Within 2-4 Days after submitting

Submit Paper Online

For Author

IJTSRD Publication

Research Area


RAPD based Molecular Characterization of Lavatera cachemiriana Cambess


Mudasir A Mir | Amrina Shafi | Insha Zahoor

https://doi.org/10.31142/ijtsrd5962



Mudasir A Mir | Amrina Shafi | Insha Zahoor "RAPD based Molecular Characterization of Lavatera cachemiriana Cambess" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-1, December 2017, pp.377-383, URL: https://www.ijtsrd.com/papers/ijtsrd5962.pdf

The exploration of genetic diversity is important to properly maintain and exploit germplasm resources and to design better strategically defined approaches in the utilization of collected germplsam so as to safeguard it from extinction. Therefore, current study was undertaken to evaluate genetic diversity of Lavatera cachemiriana collected from different geographical locations. RAPD based molecular markers were used to evaluate genetic diversity. Total DNA was isolated using CTAB method, molecular marker based polymorphism was carried out using ISSR and RAPD molecular markers, while as genetic analysis was determined in the form of RP, MI, PIC, EMR, Nei-Li’s similarity matrix using different tools and techniques. A total number of 137 DNA fragments were obtained (average- 9.13/primer) which resulted from 15 RAPD primers, among these 33 were found to be monomorphic and rest as polymorphic. The highest polymorphic bands were generated by OPT06 (9) with percentage of polymorphism as 90%. Three RAPD primers have showed number of polymorphic bands between 4-6 i.e. OPB10 (4), OPF03/OPG09 (6) with polymorphic percentage between 44.44%- 75.0%. PIC value ranged between 0-0.29 with highest value shared by OPT06 (0.23). EMR was found between 0-6.80 with highest value shared by OPG09. Nei’s genetic distance (NeiGD) and Shannon's Index (I) were found as 0.119 and 0.075 respectively. EMR was found between 0-6.80 with highest value shared by OPG09. Nei’s genetic distance (NeiGD) and Shannon's Index (I) were found as 0.119 and 0.075 respectively. Two distinct clusters (1 & 2) were obtained which formed a major cluster at 0.98 level of similarity. The cluster 1 belongs to TANG and PAMP samples and showed closer genetic relationship with 0.996 level of similarity. Therefore, the results showed that Lavatera cachemiriana possesses lower but significant levels of polymorphism among collected samples.

Lavatera kashmiriana, Genetic analysis, Molecular Characterization, Polymorphism.


IJTSRD5962
Volume-2 | Issue-1, December 2017
377-383
IJTSRD | www.ijtsrd.com | E-ISSN 2456-6470
Copyright © 2019 by author(s) and International Journal of Trend in Scientific Research and Development Journal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0)

International Journal of Trend in Scientific Research and Development - IJTSRD having online ISSN 2456-6470. IJTSRD is a leading Open Access, Peer-Reviewed International Journal which provides rapid publication of your research articles and aims to promote the theory and practice along with knowledge sharing between researchers, developers, engineers, students, and practitioners working in and around the world in many areas like Sciences, Technology, Innovation, Engineering, Agriculture, Management and many more and it is recommended by all Universities, review articles and short communications in all subjects. IJTSRD running an International Journal who are proving quality publication of peer reviewed and refereed international journals from diverse fields that emphasizes new research, development and their applications. IJTSRD provides an online access to exchange your research work, technical notes & surveying results among professionals throughout the world in e-journals. IJTSRD is a fastest growing and dynamic professional organization. The aim of this organization is to provide access not only to world class research resources, but through its professionals aim to bring in a significant transformation in the real of open access journals and online publishing.

Thomson Reuters
Google Scholer
Academia.edu

ResearchBib
Scribd.com
archive

PdfSR
issuu
Slideshare

WorldJournalAlerts
Twitter
Linkedin