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Invitro Regeneration of Arabidopsis Thaliana from Suspension and Invitro Cultures and Analysis of Regeneration and Antioxidant Enzyme Levels

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Invitro Regeneration of Arabidopsis Thaliana from Suspension and Invitro Cultures and Analysis of Regeneration and Antioxidant Enzyme Levels


Amrina Shafi | Insha Zahoor | Mudasir A Mir

https://doi.org/10.31142/ijtsrd5843



Amrina Shafi | Insha Zahoor | Mudasir A Mir "Invitro Regeneration of Arabidopsis Thaliana from Suspension and Invitro Cultures and Analysis of Regeneration and Antioxidant Enzyme Levels" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-1, December 2017, pp.37-45, URL: https://www.ijtsrd.com/papers/ijtsrd5843.pdf

Arabidopsis tissue culture is valuable for any laboratory working on this model plant. Tissue culture methodology facilitates the production of a large number of plants that are genetically identical over a relatively short growth period. Currently this in vitro regeneration system is a good system to study the mechanism by which plants show regenerative plasticity. Plant regeneration is a key technology for successful stable plant transformation, while cell suspension cultures can be exploited for metabolite profiling, kinetic study and mining. In this paper we report methods for the successful and highly efficient in vitro regeneration of plants and production of stable cell suspension lines from cotyledons of Arabidopsis thaliana. It is an easy and reproducible method of regenerating Arabidopsis plants from callus culture. A combination of 6-benzylaminopurine (BAP) and a-naphthalene acetic acid (NAA) in a Murashige and Skoog's (MS) based medium gives a high percentage of shoot formation. Further cell suspension culture were used to study the growth kinetics and also for checking level of antioxidant enzymes at different stages of culture. An expression analysis of antioxidant genes such as superoxide dismutase (SOD) and ascorbate peroxidase (APX) was also done at callus, shoot and root regeneration stage. It was found that levels and activity of these antioxidant enzymes were higher at regeneration stage, indicating antioxidant enzyme role in plant morphogenesis. Here we describe a standard protocol for regenerating Arabidopsis plants in tissue culture, and for preparing and observing samples using steriosome and bright field microscopy to study different stages of regeneration.

Arabidopsis thaliana, Suspension Culture, Callus Culture, Shoot Induction, Root Induction


IJTSRD5843
Volume-2 | Issue-1, December 2017
37-45
IJTSRD | www.ijtsrd.com | E-ISSN 2456-6470
Copyright © 2019 by author(s) and International Journal of Trend in Scientific Research and Development Journal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0)

International Journal of Trend in Scientific Research and Development - IJTSRD having online ISSN 2456-6470. IJTSRD is a leading Open Access, Peer-Reviewed International Journal which provides rapid publication of your research articles and aims to promote the theory and practice along with knowledge sharing between researchers, developers, engineers, students, and practitioners working in and around the world in many areas like Sciences, Technology, Innovation, Engineering, Agriculture, Management and many more and it is recommended by all Universities, review articles and short communications in all subjects. IJTSRD running an International Journal who are proving quality publication of peer reviewed and refereed international journals from diverse fields that emphasizes new research, development and their applications. IJTSRD provides an online access to exchange your research work, technical notes & surveying results among professionals throughout the world in e-journals. IJTSRD is a fastest growing and dynamic professional organization. The aim of this organization is to provide access not only to world class research resources, but through its professionals aim to bring in a significant transformation in the real of open access journals and online publishing.

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