Bioremediation is a degradation or transformation of contaminants into nonhazardous or less-hazardous substances using microorganism. A large number of enzymes from bacteria, fungi, and plants have been reported to be involved in the biodegradation of toxic organic pollutants. In silico approach uses variety of computational tools to predict best binding sites for pollutant degradation which permits to explore the potential of microorganisms in cleaning up the particular compound from the environment. Its broad substrate specificity offers a wide opportunity for screening pollutants in order to predict potential targets for degradation. We identified potent bacteria that can be used to accomplish bioremediation i.e. Pseudomonas putida. This study aimed to evaluate the catechol 1, 2- dioxygenase enzyme which comes in class of oxidoreductase and in sub- class of oxygenase binding affinity with the pollutant and to explore its target potential sites using docking software. The chosen pollutants were Catechol, Endosulfan, Carbaryl, Xylene, Toluene and Acrylamide. In this Pseudomonas putida wild type enzyme showed good results by endosulfan pollutant with -6.68 KJ/mol binding energy while mutated enzyme have shown better result with endosulfan with binding energy of -6.73 KJ/mol.
Bioremediation, catechol 1, 2- dioxygenase, Pseudomonas putida
International Journal of Trend in Scientific Research and Development - IJTSRD having
online ISSN 2456-6470. IJTSRD is a leading Open Access, Peer-Reviewed International
Journal which provides rapid publication of your research articles and aims to promote
the theory and practice along with knowledge sharing between researchers, developers,
engineers, students, and practitioners working in and around the world in many areas
like Sciences, Technology, Innovation, Engineering, Agriculture, Management and
many more and it is recommended by all Universities, review articles and short communications
in all subjects. IJTSRD running an International Journal who are proving quality
publication of peer reviewed and refereed international journals from diverse fields
that emphasizes new research, development and their applications. IJTSRD provides
an online access to exchange your research work, technical notes & surveying results
among professionals throughout the world in e-journals. IJTSRD is a fastest growing
and dynamic professional organization. The aim of this organization is to provide
access not only to world class research resources, but through its professionals
aim to bring in a significant transformation in the real of open access journals
and online publishing.