Evaluation of Bioactivecomponents and Antistaphylococcal Activitiesof Ethyl Acetate and Dicloromethanefractions of Moringa Oleifera Root Bark on Clinical Isolates of Methicillin Resistant Staphylococcus Aureus
This study evaluated the phytochemical components, Bioactive compounds GC MS and antistaphylocochal activities of ethyl acetate and dichloromethane fractions of Moringa oleifera root bark. MRSA Methicillin resistance staphylococcus aureus was used for the antistaphylococcal activities. The isolates from 3 different hospitals in south east geopolitical region of Nigeria were confirmed by coagulase staphylase test using Oxoid® reagents kits DR0595A . MRSA confirmation was done using Oxoid® DR0900 penicillin binding protein pbp2’ latex agglutination test kits. Pulverised Moringa oleifera root bark was extracted with methanol using Soxhlet extractor to obtain methanol crude extract ME . ME was adsorbed on Silical gel 60 200 mesh and eluted with ethyl acetate solvent to get ethyl acetate fraction EAF and also with dichloromethane solvent to get dichloromethane fraction DMF . Qualitative phytochemical analyses of the EAF and DMF were carried out using standard procedures. The antistaphylococcal activities of EAF and DMF were evaluated on the MRSA, the minimum inhibitory concentrations MICs and minimum bactericidal concentrations MBCs were recorded and compared with the standard disc antimicrobial test results. The two extract fractions were analyzed using gas chromatographic mass spectrometry GC MS for their bioactive compounds. Statistical analysis was done with ANOVA followed by Duncan post Hoc test using SPSS v 17 software. The results obtained showed DMF MIC 8.0 ± 1.1 to 10 ± 0.5 mg ml and MBC 8.0 ± 0.5 to 10 ± 0.5 mg ml EAF MIC 5.0 ± 1.1 to 8.0 ± 0.5 mg ml and MBC 5.0 ± 0.5 to 8.0 ± 0.5 mg ml. Phytochemical analysis of the extract fractions showed DMCwith concentrations high in Resins followed by steroids, fats and oil with traces of alkaloids, terpenoids and flavonoids, while EAF has high concentrations of Flavonoids, with traces of glycosides, terpenoids, steroids and carbohydrates. The GC MS analysis revealed the bioactive components from the two solvents. Ethyl acetate EAF fraction is more potent than dichloromethane fraction this indicates that the most active compound against the MRSA can be isolated from the EAF.
Activities, Antimicrobial, Bioactive, Components, Compounds, Dichloromethane, Ethyl acetate, MRSA, Phytochemical
Agboke A. A. | Attama A. A.