<b>Mutagenic effects of Ethyl Methane Sulphonate on the pollen grains of Urginea indica Kunth cytotype I</b> Urginea indica Kunth commonly called as Jangli Pyaz or jangli dungli is a medicinal plant having very high therapeutic value. Due to over exploitation, it has become vulnerable in some places. So, development of genetically advanced varieties of this plant is needed so that we can avail the benefits of this plant for a longer period of time, with great ease to mitigate several human ailments. To develop such variations in the plants through mutation breeding, screening of the plant is the most important aspect. Therefore, in this research sensitivity of Urginea indica Kunth to Ethyl Methane Sulphonate was assessed through the measurement of the mutagenic effects on pollen grains. Dose dependent decrease in the pollen fertility was observed. In addition, variation in the shape and size of the pollen grains were also observed after treatment with different concentrations. Thus, Urginea indica Kunth was found to be sensitive to Ethyl Methane Sulphonate. Urginea indica Kunth, pollen fertility, Ethyl Methane Sulphonate, mutation breeding 1048-1052 Issue-1 Volume-2 Richa Sinha