<b>Isolation, Identification and β lactamase detection of Multi Drug Resistant Acinetobacter species from Patients admitted in Intensive Care Units of Hospital</b> Acinetobacter species are ubiquitous, free living saprophytes, natural habitats are water and soil, and have been isolated from foods, arthropods, and the environment. Acinetobacter species is now a day’s very important opportunistic pathogen, responsible for the fatal health care associated Nosocomial infection with high morbidity and mortality rates. Acinetobacter species infections are more frequent in critically ill patients of intensive care units ICUs of the hospital. Such infections are widely increasing because of its ability to develop rapid resistance towards the major groups of antibiotics. That resistance is due to some specific enzymes like extended spectrum ß lactamase ESBL , carbapenemase and metallo ß lactamase. In our study isolation and identification of this fatal pathogen was carried out along with its ß lactamase detection. Total of 350 samples were studied out of which 99 isolates were obtained over a period of 18 months July 2013 December 2014 . Antibiotic susceptibility testing was done by Kirby Bauer’s disc diffusion method with commercially available antibiotic discs on Muller Hinton’s agar plates. In our study we found 17 of Acinetobacter species as multidrug resistant MDR strains with 57 ESBL positive. Acinetobacter species, Antiboigram, ESBL, MDR strains 1085-1089 Issue-1 Volume-2 Silver Panchal | Dr. Gaurav Shah | Niranjan P. Panchal