<b>Antibacterial Activity and Phytochemical Analysis of Euphorbia hirta Against Clinical Pathogens</b> Euphorbia hirta powdered plant material was extracted using two solvents distilled water and methanol. The water extract provide higher yield and also more antibacterial effectiveness than methanol extract used. The well diffusion method was used to determine the antibacterial activity against Escherchia coli, Salmonella typhi, Bacillus subtilis, pseudomonas aeruginosa, Klebsiella pneumoniae. E.hirta plant was investigated for some of their components and antimicrobial activity of their leave extract against bacteria. Phytochemical screening of the crude extracts revealed the presence of tannins, saponins, steroids, flavonoids, terpenoids, and alkaloids.In GC MS analysis, four bioactive phytochemical compounds were identified in the methanolic extract of Euphorbia hirta, the components were identified by comparing their relation indices and mass spectra Fragmentation patterns with those stored on the MS Computer library. This presence of these bioactive constituents has been linked to the antimicrobial activity of the plant material. Dry leaf extracts of E. hirta produced the highest zones of inhibition on Salmonella typhi. The herb E. hirta can be used as source of oral drugs to fight infections caused by susceptible bacteria. E.hirta, antibacterial activity, phytochemical analysis, GC MS 287-293 Issue-1 Volume-2 Shanmugam A | Subha S | Logeshwaran S