<b>Isolation, Characterization and Bioactivities of Sambucus wightiana derived Dotriacontanoic acid</b> The demand for naturally occurring bioactive molecules such as antioxidant, anticancer and antimicrobial properties is emerging globally. More and more natural products are being investigated to isolate and characterize the bioactive small molecules. Therefore, the current research work was undertaken to isolate and characterize bioactive compounds from leaf part of Sambucus wightiana. Various hyphenated spectral techniques such as Uv Vis, NMR, FT IR and MS were undertaken to investigate identity of isolated compound. The study results isolation of Dotriacontanoic acid, an important molecule that possesses fabulous medicinal properties like antioxidant, antibacterial and antioxidant. Sambucus wightiana, bioactivity, antioxidant, anti microbial,NMR, FT IR, MS 1323-1332 Issue-6 Volume-1 Mudasir A Mir | Insha Zahoor | Amrina Shafi