<b>GC MS Analysis and Evaluation of Antimicrobial Performance of Aframomum Latifolium Leaf Essential Oil from South West Nigeria</b> This study was conducted to analyze the chemical constituent and to evaluate the antimicrobial properties of the leaf essential oil of Aframomum latifolium. The leaves of this plant was subjected for hydro distillation using all glass Clevenger apparatus. The volatile oil obtained was analysed by means of gas chromatography mass spectrometry GC Ms . The oil yield was 0.08 v w. Seventeen components were identified in the leaf essential oil accounting for 99.90 of the total oil fraction. The leaf oil was dominated by oxygenated monoterpenes 51.26 and monoterpenes 36.22 . Cajeputol, an oxygenated monoterpene is the component with the highest percentage composition 45.05 , cyclofenchene 7.82 . Peperitone is the component with the lowest percentage 0.43 . The oil displayed high antimicrobial Potentials to Staphylococos aureus, Escherichia Coli, Bacillus subtilis, Pseudomonas aeruginosa, Candida albican and aspergillus niger at 100 , 50 and 25 and a moderate inhibition at 12.5 and 6.25 The result obtained from this study justifies the use of the leaf of this plant in ethnomedical practices. Aframomum latifolium, oxygenated monoterpenes, fungi, gram negative bacteria, gram positive bacteria 189-191 Issue-1 Volume-4 Ogunmola Oluranti Olagoke | Amusat Mumini A.