<b>Subchronic Effect of Yellow Root Arcangelisia Flava L Merr. Containing Berberine Boiled with Water and Brackish Water</b> Objective of this research is to examine the subchronic effect of yellow root water and brackish water decoction administered to female Wistar rats. Methods, 70 rats were divided into 7 groups the first group was treated with water as normal control the second, third and fourth groups were treated with water decoction of yellow root the fifth, sixth and seventh groups were treated with brackhish water decoction of yellow root, 1.25 g KgBW, 2.5 g KgBW and 5g KgBW respectively. The decoctions were given orally every day for 28 days. Hematological, general blood biochemical, close related histopathological and biochemical parameters of liver function were determined. HPTLC Densitometry method was used to determine the berberine contents on yellow root decoctions.The results showed that the treatment of all doses of yellow root water decoction statistically and clinically increased significant SGPT levels and interestingly they were in line with microscopic view of liver even only steatosis condition. It must be noted that the SGPT levels of rats treated with yellow root water decoctions were 64.04, 59.36 and 68.12 U L. They were significantly very high in comparison to normal range levels 16 48 U L . The berberine contents of the yellow root water decoction were 0,99 while those of yellow root brackish water decoction were 1,38 . Yellow root water decoction had toxic effect in liver function while yelow root brackish water decoction did not cause toxic effect after 28 days treatment in female Wistar rats. Subchronic toxicity, hematological, liver function, berberine, Arcangelisia flava 2569-2573 Issue-5 Volume-3 Suwijiyo Pramono | Rizqika Y. T. Paramawidhita | Marini | Saiful Bahri