Evaluation of Antifungal Susceptibility Profile of Candida Species Isolated from Female Patients Attending Aminu Kano Teaching Hospital AKTH
The increasing incidence of Candidiasis affecting the genitourinary tracts as well as the introduction of new antifungal drugs has recently encouraged the need for performing fungal susceptibility tests. The study was aimed at evaluating the antifungal susceptibility profile of Candida species isolated from female patients attending Aminu Kano Teaching Hospital AKTH , with suspected Candidal infections between August, 2012 to June, 2013. Five hundred and twenty one 521 clinical samples comprising 342 urine and 179 high vaginal swabs were cultured on Sabouraud dextrose agar. The Candida species isolated were identified to species level using Chromogenic agar and API 20 C AUX test kit. Antifungal susceptibility tests were performed using commercially prepared single antifungal disc Bioanalyse Turkey . Out of these 521 samples analyzed only 59 yielded Candida species giving the overall prevalence of 11.3 with Candida albicans 22 37.3 as the common species isolated followed by C. glabrata 19 32.2 , C. tropicalis 5 8.5 , C. krusei 3 5.1 , C. magnoliae 3 5.1 , C. lusitaniae 2 3.4 , C. parapsilosis 2 3.4 , C. famata 2 3.4 and C. guilliermondii 1 1.7 . The antifungal susceptibility test shows that 81.4 of the isolates were susceptible to ketoconazole and only 3.4 to nystatin. However, 33.9 were susceptible, 13.6 intermediate susceptible and 52.5 resistant to fluconazole. Similarly 28.8 were susceptible, 5.1 intermediate susceptible and 66.1 resistant to voriconazole. Additionally 25.4 were susceptible, 11.9 intermediate susceptible and 62.7 resistant to flucytosine. All the C. krusei isolates were completely resistant to azole drugs while C. famata were resistant to all the drugs tested. Two quality control strains of Candida namely Candida tropicalis ATCC 750 and Candida albicans ATCC 90028 were used.
Evaluation, Antifungal susceptibility Profile, Candida species
Taura, D. W. | Yakubu, G. | Panda, T. W. | Dagona, A.G.