<b>Role of Oxidative Stress in Assessment of Damage Induced by Lead Acetate in Mice Gastrocnemius Muscle</b> Heavy metal deposition has increased due to the anthropogenic activities leading to heavily polluted areas worldwide. The objective of present study was to evaluate the effect of lead acetate Pb C2H3O2 2 on antioxidant enzyme activities in gastrocnemius muscle of mice. Lead is a toxic heavy metal widely distributed in the environment due to its role in modern industry. Normal healthy looking mice showing no sign of morbidity were divided into three groups. Group I was designated as control whereas group II and group III received lead acetate having doses 10 mg kg body weight of lead acetate, daily and 150 mg kg body weight of lead acetate, weekly respectively. Study was performed at 40 and 80 days stages. Lead acetate significantly decreases antioxidant enzymes and increase oxidative stress along with muscle tissue damage. Lead acetate, gastrocnemius muscle, Antioxidant enzymes 47-50 Issue-1 Volume-3 Sushma Sharma | Anita Thakur