<b>Identification of Leaf Spot Causing Pathogen Through ITS Sequencing in Clove and it’s Management Using Bioagents, Botanicals and Chemicals</b> Clove tree is mostly grown in the hilly tracts of Tamil Nadu and Kerala. The leaves of clove seedling and grown up trees were frequently infected with leaf spot disease. The pathogen inciting leaf spot disease was isolated and identified as Colletotrichumgloeosporioides based on ITS1 5.8s, ITS 2 rDNA sequence analysis. The present study was concerned with bio agents, fungicides and botanicals which were tested against C. gloeosporioides. Among the bio agents, Trichodermaharzianum was found effective with 72.22 growth inhibition. Among the botanicals , garlic extract at 5 and 10 concentration levels was found to be effective with 56.66 and 62.66 growth inhibition respectively followed by ginger extract 48.88 and 49.66 growth inhibition. Among chemicals,propiconazole 0.1 and 0.2 was found to be the most effective fungicide in inhibiting 100 growth of C. gloeosporioides followed by hexaconazole with 87.44 and 88.55 growth inhibition at 0.1 and 0.2 respectively. Clove, Colletotrichumgloeosporioides, ITS sequencing, bio agents, botanicals and fungicides. 517-523 Issue-5 Volume-2 S. Aravindasamy | Dr. R. Kannan | Dr. M. Jayasekhar | Dr. R. Swarnapriya | Dr. V. Ramamoorthy