<b>Method Development of Methotrexate in Phosphate Buffer Solution by UV Visible Spectroscopy</b> A simple, precise, accurate, cost effective stability indicating UV Spectrophotometric method has been developed for the estimation of Methotrexate shows highest max at 303nm. Beer s law linearity response was found over a concentration range of 2 10 µg mL with good correlation coefficient r2 = 0.9987 and the values of standard deviation were satisfactory low and the recovery studies were close to 100 . The Proposed spectrophotometric method was validated as per the ICH Q1A R2 guidelines. Hence this method can be safely be employed for the routine quality control analysis of Methotrexate. Methotrexate, Method Development, UV Visible Spectroscopy 1213-1215 Issue-4 Volume-2 Nikhil Rajnani | Dr. Nalini Satish Kurup