<b>Bioanalysis An Important Tool in Drug Discovery</b> In past, the major reason causing new drug failure was the lack of proper analysis of pharmacokinetic data due to absence of any high advanced techniques but recent years witnessed various advancement in the field of analytical techniques and one of these is bioanalysis. Bioanalysis emerges as an important tool during drug discovery and development. This review article focuses on the various methods used during bioanalysis and its application in various fields. bioanalysis, sample preparation, drug extraction, validation 1273-1279 Issue-4 Volume-2 Ashutosh Badola | Sakshi Negi | Preeti Kothiyal