<b>A Study on Evaluation and Effectiveness of Herbal Hand Sanitizer and its Anti Bacterial Activity</b> An ever increasing number of customers might want Good hygiene is a minister to health, comfort, and social interactions. Current society is well aware of hygiene. Washing hands is one of the simplest, most effective ways to get rid of germs and avoid infection. Transient microorganisms can be of any type, from any source may contact the body. As hands carry out the majority of functions of the human’s body and are exposed to a variety of substances, hands hygiene plays a chief conscientiousness. Herbal plants are the unique resources of every country with potent medicinal properties pave ways towards sustainable health. In this present study, the combination of Trachyspermum copticum, Coleus aromaticus, Acorus calamus, Mentha piperita, Piper nigram, Elatteria cardamomum and Aloe barbadensis were used to formulate herbal hand sanitizer. The hand wash samples were subjected to microbial analysis. The isolated strains Yersinia, Proteus, Escherichia coli, Klebsiella, Salmonella, and Pseudomonas were characterized by their cultural characterization and confirmed using selective media. Herbal sanitizer possesses vitamin C .4 , vitamin A 0.062 and considerable microbial load. The antioxidant activity was found to be 49 and the susceptibility test showed more activity found in Yersinia sp. Proteus sp Salmonella sp. Klebseilla sp. Pseudomonas sp. E.coli. Hygiene, Hand sanitizer, Anti microbial and Anti oxidant 325-330 Issue-4 Volume-2 Dr. R. Kalaivani | Ms. S. V. Bakiyalakshmi | P. Arulmozhi