<b>Green Synthesis of ZnO Nanoparticles using Jasminum Fluminense Leaf Extract and its Spectral and Optical Characterization Studies</b> The main objective of the present study is to deal with the green synthesis of Zinc Oxide ZnO nanoparticles utilizing aqueous leaf extract of Jasminum Fluminense.` Zinc acetate Zn O2CCH3 2 H2O 2 and sodium hydroxide NaOH were used as starting materials and Jasminum Fluminense Nithyamalli leaf extract is used as precursor in the synthesis of ZnO nanoparticles. The resultant nanopowder was characterized by using Ultraviolet UV – Visible Spectroscopy, Fourier Transform Infrared Spectroscopy FTIR and Transmission Electron Microscopic TEM studies. Formation of ZnO nanoparticles has been confirmed by UV visible spectroscopy and the TEM analysis spectacles that the synthesized ZnO nanoparticles are of face centered cubic fcc structure and the size is found to be around 20 nm. FTIR spectral analysis indicated the leaf extract acts as the reducing and capping agents on the surface of ZnO nanoparticles. This simplistic and green approach may provide a useful tool to hefty extent in the synthesis of ZnO nanoparticles. These synthesized nanostructures illustrate novel applications in many fields such as cosmetics, optoelectronics, sensors, transducers and biomedical science because it is environmentally friendly and does not involve any harmful substances. Leaf extract, Nanopowder, Jasminum Fluminense and Biomedical science 258-262 Issue-4 Volume-2 S. Amudha