<b>Lab scale production and optimization of PHB Biopolymer using orange peel by Pseudomonas spp. and Bacillus spp.</b> Over the past 35 years, petrochemical derived plastics have become crucial materials in modern life. However, this oil based polymers take many years to degrade, which poses an environmental problem in some areas. An alternative for petrochemical plastic is biodegradable plastic, which is truly degradable, non polluting and economically viable which can be degraded completely into natural compounds, such as CO2, methane, H2O and biomass. Hence the objective of the current study was to produce ecofriendly and cost effective PHB from fruit waste i.e. Orange peels by Bacillus species and Pseudomonas species and to optimize the process at different parameters like Carbon source 15grams and 20grams and Nitrogen source 4grams and 6grams . It was observed that Pseudomonas species showed the highest production of PHB. It was observed that by increasing the concentration of both Carbon and Nitrogen sources the production of PHB was strikingly enhanced where Bacillus species showed the highest production of PHB when grown under production media containing 20gms of Carbon and 6gms of Nitrogen sources. The presence of blue black granules confirmed that the chosen organisms produced PHB. PHB, Bacillus, Pseudomonas, Orange Peels 638-642 Issue-3 Volume-2 Mayur N. Rohi | Shivani Parwar | Ashok V. Gomashe