Diabetes mellitus is marked by chronic hyperglycemia due to defective insulin secretion or action. This study evaluated the in vitro glycemic control potential of Dia Care Kwath Juice, a novel polyherbal liquid formulation, manufactured by Multani Pharmaceuticals Ltd., comprising traditional herbs are Eugenia jambolana, Momordica charantia, Gymnema sylvestre, Trigonella foenum graecum, Tinospora cordifolia, Azadirachta indica, Emblica officinalis, Curcuma longa, and Pterocarpus marsupium. Glycemic control potential was evaluated through a amylase and a glucosidase inhibition assays, using acarbose as the standard, alongside glucose uptake in differentiated L6 myotubes. Cytotoxicity was assessed via MTT assay, with IC50 values derived from nonlinear regression in GraphPad Prism. Dia Care Kwath Juice exhibited dose dependent enzyme inhibition. It achieved significant a amylase inhibition IC50 6.482 , maximum inhibition 85.07 and potent a glucosidase inhibition IC50 3.012 , maximum inhibition 91.37 . In L6 myotubes, the formulation markedly boosted glucose uptake, peaking at 2 concentration with a 2.83 fold increase over basal levels. MTT assay indicated concentration dependent cytotoxicity IC50 7.69 in L6 cells , confirming viability at lower doses suitable for functional assays. These results demonstrated that Dia Care Kwath Juice s robust glucose lowering and insulin sensitizing properties, positioning it as a promising adjunct for managing healthy blood sugar balance. a amylase inhibition, a glucosidase inhibition, Acarbose, Doxorubicin, Glucose uptake assay, IC50 value, MTT assay, polyherbal formulation. 380-391 Issue-3 Volume-10 Dr. Ruchi Srivastava | Mohammad Amaan Khan | Nakul Multani | Dr. Yogisha Shivanna