The present study was undertaken in order to develop an efficient in vitro propagation protocol for Petunia hybrid using leaf as an explant. The effects of different concentrations of five different carbon sources i.e., sucrose, glucose, mannose, arabinose and maltose was investigated on shoot regeneration of Petunia hybrid. Shoot regeneration occurred on MS medium supplemented with sucrose, glucose and maltose. No growth was obtained on MS medium when mannose and arabinose were used as carbon source. Best shoot regeneration with maximum shoot length and maximum number of shoots was obtained on MS medium supplemented with 3% carbon within 11 days with 70% culture response. Shoot regeneration was also obtained on MS medium supplemented with glucose and maltose within 18 and 24 days with 50 and 30% culture response respectively.
Explant, Maltose, MS medium, Petunia hybrid, Regeneration
International Journal of Trend in Scientific Research and Development - IJTSRD having
online ISSN 2456-6470. IJTSRD is a leading Open Access, Peer-Reviewed International
Journal which provides rapid publication of your research articles and aims to promote
the theory and practice along with knowledge sharing between researchers, developers,
engineers, students, and practitioners working in and around the world in many areas
like Sciences, Technology, Innovation, Engineering, Agriculture, Management and
many more and it is recommended by all Universities, review articles and short communications
in all subjects. IJTSRD running an International Journal who are proving quality
publication of peer reviewed and refereed international journals from diverse fields
that emphasizes new research, development and their applications. IJTSRD provides
an online access to exchange your research work, technical notes & surveying results
among professionals throughout the world in e-journals. IJTSRD is a fastest growing
and dynamic professional organization. The aim of this organization is to provide
access not only to world class research resources, but through its professionals
aim to bring in a significant transformation in the real of open access journals
and online publishing.