An efficient in vitro propagation protocol has been developed in Petunia hybrida using nodes as explants. Both direct as well as indirect shoot regeneration was achieved. Direct shoot regeneration was obtained on MS basal medium after 7 days of inoculation with 50% culture response. Medium containing BAP 0.5 mg/l resulted in direct shoot regeneration after 13 days of inoculation with 50% culture response. Best results were obtained on MS medium supplemented with BAP 1 mg/l which resulted in indirect shoot regeneration in 70% cultures after 5 average number of days. Indirect shooting with 20% culture response was also obtained on MS medium containing BAP 2 mg/l after 10 days. However, medium containing Kn 1 mg/l resulted only in direct shoot regeneration after 5 days of inoculation with 60% culture response.
Petunia hybrida, Solanaceae, Nodal explant, MS medium, BAP, Kn
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