Callus culture of Azadirachta indica employing its stem

Saurish Sarkar

Title:
Callus culture of Azadirachta indica employing its stem

Authors:
Saurish Sarkar
DOI:
https://doi.org/10.31142/ijtsrd10911

Cite This Article :

Saurish Sarkar "Callus culture of Azadirachta indica employing its stem" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-3, April 2018, pp.318-323, URL: https://www.ijtsrd.com/papers/ijtsrd10911.pdf

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Abstract :
Azadirachtin,an insecticidal active ingredient present in neem tree i.e.Azadirachta indica A. Juss.seeds and they varies considerably due to environmental factors also for genetic reasons. Furthermore, the tree does not grow in moderate climates and their germination ratio is very less. It seems appropriate, therefore, to employ tissue culture techniques for the production of Azadirachtin in order to obtain constant amounts of standardized quality. Here we try to create callus, an unspecialized, unorganized, growing and dividing mass of cells through which mass cultivation further initiate. The best media generally we use is MS Media along with different concentrations of hormones i.e. Auxins(BAP) and Cytokinin (Kinetin), employing Azadirachta indica stem.

Keywords :
Micro propagation, Callus, MS media, Auxin, Cytokinin, Root, Shoot

Publication Details:

Unique Identification Number : IJTSRD10911

Published In : Volume-2 | Issue-3, April 2018

Page Number(s) : 318-323

Publisher Name : IJTSRD | www.ijtsrd.com | E-ISSN 2456-6470

Copyright © 2019 by author(s) and International Journal of Trend in Scientific Research and Development Journal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0)

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